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KMID : 1007520070160050858
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Food Science and Biotechnology
2007 Volume.16 No. 5 p.858 ~ p.862
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Protective Effect of Schizonepeta tenuifolia Briquet Extracts on Oxidative DNA Damage in Human Leucocytes and on Hydrogen Peroxide-induced Cytotoxicity in PC12 Cells
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Yoon Mi-Young
Lee Hyun-Jin
Lee Bo-Bae
Lee Sang-Myeon
Kim Ju-Young
Kim Yong-Seong
Park Eun-Ju
Park Hae-Ryong
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Abstract
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The present study was conducted to examine the antioxidant activities and neuroprotective effects of methanolic extracts from Schizonepeta tenuifolia Briquet (STE). STE (100 μg/mL) showed 43.33 μM of total phenolic content, 64.43% of radical scavenging activity, and 0.157 of reducing power. In addition, the effect of STE on H2O2-induced DNA damage in human leucocytes was evaluated by the comet assay, where STE was a dose dependent inhibitor of DNA damage induced by 200 μM of H2O2. The protective effect of STE against H2O2-induced oxidative damage on PC12 cells was investigated by an 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) reduction assay and lactate dehydrogenase (LDH) release assays. After 2 hr of cell exposure to H2O2 (500 μM), a marked reduction in cell survival was observed. However, this reduction was significantly prevented by 1-50 μg/mL of STE. Therefore, these results suggest that STE could be a new antioxidant candidate against neuronal diseases.
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KEYWORD
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Schizonepeta tenuifolia Briquet, antioxidant, human Leucocyte, hydrogen peroxide, PC12 cell
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